187+ Series B Startups — $12.7B Raised, $53M Median Round

Series B funding in the 2025-2026 window is concentrated among companies demonstrating strong unit economics and a clear path to profitability. Our dataset includes 141 Series B companies that raised a combined $12.7B, with a median round of $53M — well above the overall $18M median. AI dominates Series B activity: 37 AI companies raised $7.0B at a striking $90M median, reflecting the capital intensity of scaling AI infrastructure and enterprise sales teams. Healthcare Series B (14 companies, $645M, $30M median) and FinTech Series B (11 companies, $520M, $45M median) round out the top sectors. The bar has shifted decisively from growth-at-all-costs to efficient growth — Series B investors want to see a CAC payback period under 18 months and gross margins above 70%. Companies raising Series B are typically generating $5-15M in ARR with 100%+ net dollar retention. For GTM teams, Series B companies are premium prospects. They have substantial budgets ($53M median in fresh capital), established processes, and dedicated procurement functions. They are scaling sales teams from 10 to 50+ reps, expanding to new markets, and investing heavily in infrastructure. Outreach should be multi-threaded across VP-level stakeholders rather than founder-direct.